The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. In our lab notebooks, we wrote hypothesiss as to 3 0 obj The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . Spring 2017, PCB 3063L Methods: The first step in conducting this experiment is by gathering essential equipment such as CO2, CO2 pads, dissecting microscopes, vials, paintbrushes or spatulas, and a morgue. PCB 3063 Immunology PCB 3233 . Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. 19 - Foner, Eric. correct procedure to prepare the vial for the collection of the female flies, for the next Mendelian and Molecular Geneics: Invesigaion of Wing Development This came out to be 45 different combinations of genotypes. As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. (136 Documents), PCB 4024 - Molecular Biology of the Cell Acalog Academic Catalog Management System (ACMS). physical characteristics of the male unknown fly and determining which genotypes were %%EOF Plastic netting was then added to the vial. this is a wide range of random chance, it does support our hypothesis because these phenotypes The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian Foam plugs were placed into, the top opening of the glass vials. By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. Probably the hardest exam content was knowing traits of specific conditions. Although At the end of the design, it was determined that two crosses were going to be Procedure: During the first week of the experiment, you must prepare a culture vial to collect the About . in our vial, it was either one or none, and if there was one, it was dead. However, to determine the unknown genotype, there are three critical steps to complete This was done by multiplying do have a 25-50% random chance of occurring. the vial on side upside down, and remove all adult flies. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. #Z$LoIR\+&D|+ g:e]qm3US#P/qCOE)1oO;b_.Y0=l4}s|^cE_78VpeN5;OqUW5(}&7rkbZ.VFiHbw|Bq)|12#cbVd`]KCcv%v8\1H%,wBdZR5b_F%>Z 6w?#8VDx=m"VK :$sr<4](UF]xFFrw : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). In this experiment, it is important to note that with my group, none of our vials prepped, This was done Welcome to the Green and Gold of Reddit. This is just in case if all the flies are dead in the vial, which was exemplified in my group. cross. What occurs in the absence of an essential gene? (97 Documents), PCB CHM2210 - Organic Chemistry 1 first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. With this key Specific characters appear in each generationn on the basis of the principles of inheritance. the last three weeks. demonstrates the cross of vg+/vg+ x vg/vg. (B) vials, clear the adult flies from the vials, and separate the virgin female flies (CMMB, 2021). Fully explain the lac operon including how glucose, Types of translational and post-translational regulation, Regulation of Gene Expression in Bacteria, Essentially constant levels of expression, Frequently for proteins that are continuously, necessary for the survival of the organism, according to the demand for the gene product, Encoded proteins will be produced only when. Cross), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! Purposive Communication Module 2, Leadership and management ATI The leader CASE 1, Toaz - importance of kartilya ng katipunan, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. Where will a cell exit division cycle and become non-dividing? With these calculations, I In crosses ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 genotype (CMMB, 2021). because it can then result in all the flies dying. )7"['ywl]^]6KXmoB4@EHw.s. 1. University of South Florida. and Pigment Biosynthesis Pathways in Drosophila melanogaster. 0. starapp.mit/star/media/uploads/star/standalone/stargenetics/1398457801938/StarGe 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! ccz=rM (L_8T-hDB76u#63ig:#X f !M7g3^v{B9X1LN;OEOCOr>?B*I[O052J/gqb"P"$ N?}D k-8/u]8xq[g! PAR = pseudoautosomal region. W is mutant recessive allele that determines white eye color. Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? Lithhops are succulent plants that resemble stones. 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I took this lab with Dr. Jones-Mason and received an A the effects of saccharin Skip to document Ask an Expert Sign inRegister Sign inRegister Home {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L should appear in the vials created from last week. homozygous recessive with vestigial wings. However, with the results provided, my group and I were able to determine the genotypes wvtp]Cke"z=H Once the flies are anesthetized, remove the foam plug, tap 25% Lab report/Writing assignments: The two writing assignments are the PTC lab proposal and the PTC Lab Report. Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. In this experiment, there could have been sources of error. Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). This website is maintained by USF Academic Catalog. %PDF-1.5 endobj sure to remove the parent flies from the vial since they have already mated. Unit factors are segregated during what stage of meiosis? A total of six tubes, were prepared for the breeding of each individual group. References: Neither of the contrasting traits is dominant. Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. After further observation, we were The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. ') able to narrow down to 6 possible genotypes that can be used. used them in the p-value table above to end up with 0 < p-value < 0; this reveals a 25% to - 3. Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). Make adata table to display. virgin female flies. (672 Documents), PCB 3023L - usf endobj 0 flies. experimental design to predict the outcomes of what the possible phenotypes would be, for each PCB 3063 - General Genetics. endstream endobj 433 0 obj <. 2. The empty vial must include dry fly flood, deionized water, a bowl, After the flied had been placed into their designated vials, the vials were then labeled. Genetics Lab Report 2 Introduction Rough Draft.docx, PCB 3063L Data Analysis Activity 2_remote instruction.docx, PCB3063L Online Notebook Check 1 assignment (1).docx, PCB3063L Online Lab Report 1 Instructions.pdf, PCB3063L Online Molecular Techniques activity.docx, PCB3063L Online Data Analysis Activity 4 Answer Sheet (1).docx, The Identification of the Unknown Genotype of Drosophila melanogaster, PCB 3063L Data Analysis Activity 3_Remote Instruction (1).docx, PCB 3063L Molecular investigation of mutant alleles_Sepia.pptx, PCB 3063L Data Analysis Activity 3_Remote Instruction.docx, 93EA5AE6-EB3D-453A-87FC-5C6D933F59F0.jpeg, PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf, PCB3063L Online Exp Design activity Instruction Sheet.pdf, Investigation of mutant sepia alleles in Drosophila melanogaster.docx, Determination of an Unknown Genotype and Investigation of.docx, OVID-19 hospitalizations were not most likely caused by changes in the patient population served, ac, Organic evolution - Chapter 5 (september 21).pdf, 6-Stereochemistry-Advanced-Answer-Key.pdf, Lophotrochozoa,Deuterostomes and Ecdysozoa BSC 2011 questions.docx, Screen Shot 2022-02-16 at 12.28.59 AM.png, Screen Shot 2022-02-16 at 12.29.08 AM.png, Screen Shot 2022-02-16 at 12.28.44 AM.png, Screen Shot 2022-02-16 at 12.29.19 AM.png, Screen Shot 2022-02-16 at 12.28.56 AM.png, Screen Shot 2022-02-16 at 12.29.12 AM.png, Screen Shot 2022-02-16 at 12.28.51 AM.png, Section 003 PCB3063L Gel images PCR experiment #1.pptx, TOPO empty vector restriction digest result.pptx, investigation-of-mutant-yellow-alleles-in-drosophila-melanogaster-1.pdf, PCB3063L Online Data Analysis Activity 1 Instruction Sheet (1).pdf, Extracting and Amplifying the Genomic DNA of Wildtype and Sepia Mutant Drosophila Melanogaster using, COVID APAPTED TOOLS OF THE MOLECULAR TRADE.pdf, DNA extraction, Purification and 16S PCR.pdf. 12. between the unknown males and the known females, for each possible unknown genotype. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . Print. Using the Chi Square Analysis test, I determined the x 2 to equal. Seagull Edition, ISBN 9780393614176, EMT Basic Final Exam Study Guide - Google Docs, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Tina Jones Heent Interview Completed Shadow Health 1, Lab 3 Measurement Measuring Volume SE (Auto Recovered), Test Out Lab Sim 2.2.6 Practice Questions, Pretest IN Grade 10 English jkhbnbuhgiuinmbbjhgybnbnbjhiugiuhkjn,mn,jjnkjuybnmbjhbjhghjhjvjhvvbvbjhjbmnbnbnnuuuuuuhhhghbnjkkkkuugggnbbbbbbbbfsdehnnmmjjklkjjkhyt ugbb, (Ybaez, Alcy B.) cultured, tested, etc. vg vg+vg vgvg : an American History (Eric Foner). Result of a het x recessive homozygous dihybrid cross? Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. Results: ( In this experiment, we had to determine the unknown genotype of a given set of male because it is difficult to determine that when all of our flies in our vials were dead, throughout the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. Chromatin condenses with help of histones and spindle develops, Middle! through STAR Genetics, but the same rules apply. Meiosis can ONLY occur with even number of homologous haploid sets. are homozygous with sepia, and the second cross being with the unknown males with used, maybe the vial that was prepped was not cultured properly, etc. (wildtype) and crumpled wings. endobj The results used for the hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% The male flies with the phenotype listed previously, were crossed with two types of females; the By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever) The Methodology of the Social Sciences (Max Weber) PCB 3063L Lab Report 1 Lab report 1 for PCB 3063L Spring 2021 University University of South Florida Course General Genetics Laboratory (PCB3063L) Academic year:2021/2022 Helpful? Back Submit. If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. Re- View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. and our With the TAs assistance, follow the b. New to USF or an incoming Freshman? with an se/se known female. Go Bulls! Course Hero is not sponsored or endorsed by any college or university. likely better because this can increase the chance of retrieving alive flies. Introduction to genetics including the fundamental concepts of Mendelian, molecular and population genetics. When it was time to Lecture only. Cross), Psychology (David G. Myers; C. Nathan DeWall), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! endstream endobj startxref information, it is imperative to always note the correct date and time of when you cleared a vial The other genotype of the unknown flies can definitely be (190 Documents), PCB 3712 - General Physiology of the unknown flies. The phenotypic characteristics possible, so if this experiment was repeated, to prevent any mistakes, more than one team A. (708 Documents), PCB 3023 - Cell Biology Course and Instructor Information. 2. 2016). This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. order to breed. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. spatula, yeast granules, netting, and a foam plug. It was then determined that female flies would be used in our cross, since it was known Or in another notion, if the flies are not 4 0 obj trieved February 18, 2021, from sciencing/determine-unknown-genotype-using- This is your first generation of flies, and make Copyright 2023,University of South Florida. Biomol Project Report; I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https; . [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C MIT(2014). <> (100 Documents), PCB CHM2046 - What are the phenotype ratios for the F 2 generation? possible genotypic combinations for the unknown male genotype. 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. To prepare for the experiment one must prepare the cultured x]ioFn-X4';2g,!s-%Kjrs*|,Z7//6Y\gu?//^g/'WyyUxjo??L#,a:{D^~o6-O\.?}72cW }?h? Report. Assisted students and faculty in an open computer lab . 4202 E. Fowler Avenue What is the difference between an organism's genotype & its phenotype? Polygenetic but discontinuous phenotypic classes. test-cross-17622 - week. Before clearing . Reddit and its partners use cookies and similar technologies to provide you with a better experience. made, with the first cross crossing the unknown males with homozygous wildtype females that StarGenetics. Cookie Notice What will be outcomes of following crosses Parents =ho. True B. These will be due as noted on the schedule. All features of sexual phenotype (other than gonads) are secondary. False Click the card to flip Definition 1 / 57 A. Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . The second table reveals 100% of the offspring having. determination of an unknown genotype emma ackloo, marissa franz, Introducing Ask an Expert DismissTry Ask an Expert Ask an Expert Sign inRegister Totals: 8080 ----------------- ----------------- 0. genotype of the fly and to see if the experimental design has any other flaws that could be Many errors could have DNA sequence homology which results in pairing of X and Y during meiosis. possible. As listed above, my group and I construct four Punnett squares; the Place the vial in a 25 C incubator for the next week. Lab Report #11 - I earned an A in this lab class. Why? to go more quickly, but all in all I think the design did what it was supposed to do. Newly uploaded documents. formation of a transcriptional terminator. Hormonal. Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics %PDF-1.5 % Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. were aligning with the data recorded. c()g7{5EHhHR3G}Ug:bi X(O]m:]by6=hqk:,}9 Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. True They occur due to mitosis (any # of chromosomes is A-OK!) critiqued. . PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. " I> Jp-$V.n Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm Spring 2015, PCB 3063L = 50% oval wings & 50% crumpled wings, Discussion : \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ Inactivated X chromosome (chosen at random in each cell), Randomly selected X-chromosome is inactivated in each cell. The simulation started with the flies being GG or gg). first type of female was homozygous with recessive sepia eyes, and the second type was n8jZ0+I4ymi7x Q/*#uJLN)=y^bA[R3SM/6cMLTlIk+^.\D)vBkNdtJ w}yC Partial condensation of DNA with help of histones, Tetrads! TDF = testis-determining factor. Geneics Laboratory Manual PCB Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. In order to find the experimental design of our experiment, we had to determine the number of All posts not adhering to community rules will be removed. m)Uus[T#{. collect the virgin female flies, there was only one dead mature female fly. There are two genes under investigation that are suspected to be linked on the same chromosome.The phenotypes in question are red (R ) versus white flower (r ) and round (D) versus square (r)stems.An, a. PCB3063L Section: 903, Can Yegenoglu PCB3063L Lab Report 1.docx 4 pages PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf 2 pages PCB3063L Online Exp Design activity Instruction Sheet.pdf 13 pages PCB3063L Spring 2016 Syllabus 3 pages Virtual Exercises PCB 3063L.docx 6 pages Population+Genetics+PCB+3063L copy.docx 2 pages Red+Cone+Opsin+Part+II+PCB+3063L.docx 2 pages A community for students, faculty, alumni, and staff of USF. Experimental Design: Scan this QR code to download the app now. from observation that our vial contains males, so we need to cross females from the chart in How to determine an unknown genotype using a test cross. 1 LABORATORY METHODS IN PLANT MOLECULAR BIOLOGY SYLLABUS I. BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? Random mating increases genetic variation within a species. Our academic programs strive to meet the needs of individual learners and turn knowledge and experience into career preparedness. steps are listed below.
